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Umeå universitet
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Personalbild Andrei Chabes

Andrei Chabes

Professor i medicinsk kemi

dNTP: genomets byggstenar

Kontakt

E-post
andrei.chabes@umu.se
Telefon
073-620 54 46

Verksam vid

Anknytning
Professor vid Institutionen för medicinsk kemi och biofysik Roll: Katalogansvarig
Plats
KBC building, H6, Campus, Umeå, A6-42-21 Umeå universitet, 901 87 Umeå
Anknytning
Anknuten som övrig/annan befattning till Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM) Roll: Föreståndare
Plats
Umeå universitet, 901 87 Umeå

DNA byggs ihop av fyra olika byggstenar - dATP, dTTP, dGTP, och dCTP- som gemensamt även kallas dNTP. Målet med mitt projekt är att förstå hur förändringar i mängden DNA-byggstenar i cellen påverkar genomstabilitet, åldrande och uppkomsten av sjukdomar, samt utveckla metoder för att bota dessa sjukdomar genom riktade förändringar av DNA-byggstensnivåerna.

Våra senaste publikationer:

Das B, Mishra P, Pandey P, Sharma, S and Chabes, A (2022) dNTP concentrations do not increase in mammalian cells in response to DNA damage. Cell Metabolism 34:1895-1896 

Wanrooij PH, Tran P, Thompson LJ, Carvalho G, Sharma S, Kreisel K, Navarrete C, Feldberg AL, Watt DL, Nilsson AK, Engqvist MKM, Clausen AR and Chabes A (2020) Elimination of rNMPs from mitochondrial DNA has no effect on its stability. PNAS 117:14306-14313  

Sushma S, Koolmeister C, Tran P, Nilsson AK, Larsson NG and Chabes A (2020) Proofreading deficiency in mitochondrial DNA polymerase does not affect total dNTP pools in mouse embryos. Nature Metabolism 

doi.org/10.1038/s42255-020-0264-z

Alla publikationer i PubMed (senaste först) och i Google Scholar (mest citerade först).

 

 

Developmental signals control chromosome segregation fidelity during pluripotency and neurogenesis by modulating replicative stress
Nature Communications, Springer Nature 2024, Vol. 15, (1)
de Jaime-Soguero, Anchel; Hattemer, Janina; Bufe, Anja; et al.
Altered dNTP pools accelerate tumor formation in mice
Nucleic Acids Research, Oxford University Press 2024, Vol. 52, (20) : 12475-12486
Tran, Phong; Mishra, Pradeep; Williams, Leonard G.; et al.
Impairment of the non-catalytic subunit Dpb2 of DNA Pol ɛ results in increased involvement of Pol δ on the leading strand
DNA Repair, Elsevier 2023, Vol. 129
Dmowski, Michal; Makiela-Dzbenska, Karolina; Sharma, Sushma; et al.
Quantitative analysis of nucleoside triphosphate pools in mouse muscle using hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry detection
Mitochondrial DNA: methods and protocols, New York: Humana Press 2023 : 267-280
Sharma, Sushma; Kong, Ziqing; Jia, Shaodong; et al.
NME6: ribonucleotide salvage sustains mitochondrial transcription
EMBO Journal, John Wiley & Sons 2023, Vol. 42, (18)
Wanrooij, Paulina H.; Chabes, Andrei
Chl1 helicase controls replication fork progression by regulating dNTP pools
Life Science Alliance, Vol. 5, (4)
Batté, Amandine; van der Horst, Sophie C.; Tittel-Elmer, Mireille; et al.
dNTP concentrations do not increase in mammalian cells in response to DNA damage
Cell Metabolism, Elsevier 2022, Vol. 34, (12) : 1895-1896
Das, Biswajit; Mishra, Pradeep; Pandey, Praveen; et al.
Increased contribution of DNA polymerase delta to the leading strand replication in yeast with an impaired CMG helicase complex
DNA Repair, Elsevier 2022, Vol. 110
Dmowski, Michal; Jedrychowska, Malgorzata; Makiela-Dzbenska, Karolina; et al.
Isocratic HPLC analysis for the simultaneous determination of dNTPs, rNTPs and ADP in biological samples
Nucleic Acids Research, Oxford University Press 2022, Vol. 50, (3)
Ranjbarian, Farahnaz; Sharma, Sushma; Falappa, Giulia; et al.
High density of unrepaired genomic ribonucleotides leads to Topoisomerase 1-mediated severe growth defects in absence of ribonucleotide reductase
Nucleic Acids Research, Oxford Academic 2020, Vol. 48, (8) : 4274-4297
Cerritelli, Susana M; Iranzo, Jaime; Sharma, Sushma; et al.
SAMHD1 agit sur les fourches de réplication bloquées pour empêcher l’induction d’interféron: [SAMHD1 acts at stalled replication forks to prevent interferon induction]
Comptes rendus. Biologies, Académie des Sciences 2020, Vol. 343, (1) : 9-21
Coquel, F.; Silva, M. J.; Techer, H.; et al.
SAMHD1 Limits the Efficacy of Forodesine in Leukemia by Protecting Cells against the Cytotoxicity of dGTP.
Cell Reports, Elsevier 2020, Vol. 31, (6)
Davenne, Tamara; Klintman, Jenny; Sharma, Sushma; et al.
Mec1 Is Activated at the Onset of Normal S Phase by Low-dNTP Pools Impeding DNA Replication
Molecular Cell, Elsevier 2020, Vol. 78, (3) : 496-410.e4
Forey, Romain; Poveda, Ana; Sharma, Sushma; et al.
Inactivation of folylpolyglutamate synthetase Met7 results in genome instability driven by an increased dUTP/dTTP ratio
Nucleic Acids Research, Oxford University Press 2020, Vol. 48, (1) : 264-277
Schmidt, Tobias T; Sharma, Sushma; Reyes, Gloria X; et al.
Proofreading deficiency in mitochondrial DNA polymerase does not affect total dNTP pools in mouse embryos
Nature Metabolism, Nature Publishing Group 2020, Vol. 2, (8) : 673-675
Sharma, Sushma; Koolmeister, Camilla; Tran, Phong; et al.
Elimination of rNMPs from mitochondrial DNA has no effect on its stability
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences 2020, Vol. 117, (25) : 14306-14313
Wanrooij, Paulina H.; Tran, Phong; Thompson, Liam J.; et al.
dNTPs and maintenance of genome stability
FEBS Open Bio, WILEY 2019, Vol. 9 : 21-21
Chabes, Andrei
The absence of the catalytic domains of Saccharomyces cerevisiae DNA polymerase ϵ strongly reduces DNA replication fidelity
Nucleic Acids Research, Oxford University Press 2019, Vol. 47, (8) : 3986-3995
Garbacz, Marta A.; Cox, Phillip B.; Sharma, Sushma; et al.
Dinucleotide Degradation by REXO2 Maintains Promoter Specificity in Mammalian Mitochondria
Molecular Cell, Elsevier 2019, Vol. 76, (5) : 784-+
Nicholls, Thomas J.; Spåhr, Henrik; Jiang, Shan; et al.
A geographically matched control population efficiently limits the number of candidate disease-causing variants in an unbiased whole-genome analysis
PLOS ONE, Public Library of Science 2019, Vol. 14, (3)
Rentoft, Matilda; Svensson, Daniel; Sjödin, Andreas; et al.
A genetic screen pinpoints ribonucleotide reductase residues that sustain dNTP homeostasis and specifies a highly mutagenic type of dNTP imbalance
Nucleic Acids Research, Oxford University Press 2019, Vol. 47, (1) : 237-252
Schmidt, Tobias T.; Sharma, Sushma; Reyes, Gloria X.; et al.
De novo dNTP production is essential for normal postnatal murine heart development
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology 2019, Vol. 394, (44) : 15889-15897
Tran, Phong; Wanrooij, Paulina H.; Lorenzon, Paolo; et al.
Ribonucleotides in mitochondrial DNA
FEBS Letters, John Wiley & Sons 2019, Vol. 593, (13) : 1554-1565
Wanrooij, Paulina H.; Chabes, Andrei
A recurrent cancer-associated substitution in DNA polymerase ε produces a hyperactive enzyme
Nature Communications, Nature Publishing Group 2019, Vol. 10
Xing, Xuanxuan; Kane, Daniel P.; Bulock, Chelsea R.; et al.
Mrc1 and Rad9 cooperate to regulate initiation and elongation of DNA replication in response to DNA damage
EMBO Journal, Wiley-VCH Verlagsgesellschaft 2018, Vol. 37, (21)
Bacal, Julien; Moriel-Carretero, María; Pardo, Benjamin; et al.
SAMHD1 acts at stalled replication forks to prevent interferon induction
Nature, Vol. 557, (7703) : 57-61
Coquel, Flavie; Silva, Maria-Joao; Técher, Hervé; et al.
Simultaneous determination of ribonucleoside and deoxyribonucleoside triphosphates in biological samples by hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry
Nucleic Acids Research, Oxford University Press 2018, Vol. 46, (11)
Kong, Ziqing; Jia, Shaodong; Chabes, Anna Lena; et al.
Separable roles for Mec1/ATR in genome maintenance, DNA replication, and checkpoint signaling
Genes & Development, Vol. 32, (11-12) : 822-835
Lanz, Michael Charles; Oberly, Susannah; Sanford, Ethan James; et al.
Rtt105 functions as a chaperone for replication protein A to preserve genome stability
EMBO Journal, Wiley-VCH Verlagsgesellschaft 2018, Vol. 37, (17)
Li, Shuqi; Xu, Zhiyun; Xu, Jiawei; et al.
Acute Smc5/6 depletion reveals its primary role in rDNA replication by restraining recombination at fork pausing sites
PLOS Genetics, PUBLIC LIBRARY SCIENCE 2018, Vol. 14, (1)
Peng, Xiao P.; Lim, Shelly; Li, Shibai; et al.
Upregulation of dNTP Levels After Telomerase Inactivation Influences Telomerase-Independent Telomere Maintenance Pathway Choice in Saccharomyces cerevisiae
G3: Genes, Genomes, Genetics, Vol. 8, (8) : 2551-2558
van Mourik, Paula M; de Jong, Jannie; Sharma, Sushma; et al.
A mechanism for preventing asymmetric histone segregation onto replicating DNA strands
Science, American Association for the Advancement of Science 2018, Vol. 361, (6409) : 1386-+-
Yu, Chuanhe; Gan, Haiyun; Serra-Cardona, Albert; et al.
Checkpoint Kinase Rad53 Couples Leading- and Lagging-Strand DNA Synthesis under Replication Stress
Molecular Cell, Elsevier 2017, Vol. 68, (2) : 446-455
Gan, Haiyun; Yu, Chuanhe; Devbhandari, Sujan; et al.
Shortage of dNTPs underlies altered replication dynamics and DNA breakage in the absence of the APC/C cofactor Cdh1
Oncogene, Macmillan Publishers Ltd. 2017, Vol. 36, (42) : 5808-5818
Garzón, J.; Rodríguez, R.; Kong, Ziqing; et al.
Yeast DNA polymerase ζ maintains consistent activity and mutagenicity across a wide range of physiological dNTP concentrations
Nucleic Acids Research, Vol. 45, (3) : 1200-1218
Kochenova, Olga V; Bezalel-Buch, Rachel; Tran, Phong; et al.
Rnr1, but not Rnr3, facilitates the sustained telomerase-dependent elongation of telomeres
PLOS Genetics, Vol. 13, (10)
Maicher, André; Gazy, Inbal; Sharma, Sushma; et al.
Inosine triphosphate pyrophosphatase enhances the effect of ribavirin on hepatitis C virus cell culture infection
Journal of Hepatology, ELSEVIER SCIENCE BV 2017, Vol. 66, (1) : S321-S321
Nystrom, K.; Pettersson, G.; Wanrooij, Paulina; et al.
Alterations in cellular metabolism triggered by URA7 or GLN3 inactivation cause imbalanced dNTP pools and increased mutagenesis
Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, (22) : E4442-E4451
Schmidt, Tobias T; Reyes, Gloria; Gries, Kerstin; et al.
Ribonucleotides incorporated by the yeast mitochondrial DNA polymerase are not repaired
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences 2017, Vol. 114, (47) : 12466-12471
Wanrooij, Paulina H.; Engqvist, Martin K. M.; Forslund, Josefin M. E.; et al.
The mutation spectrum in genomic late replication domains shapes mammalian GC content
Nucleic Acids Research, Vol. 44, (9) : 4222-4232
Kenigsberg, Ephraim; Yehuda, Yishai; Marjavaara, Lisette; et al.
Hydroxyurea-Mediated Cytotoxicity Without Inhibition of Ribonucleotide Reductase
Cell Reports, Vol. 17, (6) : 1657-1670
Liew, Li Phing; Lim, Zun Yi; Cohen, Matan; et al.
Heterozygous colon cancer-associated mutations of SAMHD1 have functional significance
Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, (17) : 4723-4728
Rentoft, Matilda; Lindell, Kristoffer; Tran, Phong; et al.
Genome-wide analysis of the specificity and mechanisms of replication infidelity driven by imbalanced dNTP pools
Nucleic Acids Research, Vol. 44, (4) : 1669-1680
Watt, Danielle L.; Buckland, Robert J.; Lujan, Scott A.; et al.
Myc-dependent purine biosynthesis affects nucleolar stress and therapy response in prostate cancer
Oncotarget, Vol. 6, (14) : 12587-12602
Barfeld, Stefan J; Fazli, Ladan; Persson, Margareta; et al.
Acute sensitivity of DNA replication to reduction in dNTP pools following Mec1ATR inactivation
Yeast, Wiley-Blackwell 2015, Vol. 32, (Suppl. 1) : S56-S56
Earp, Caroline; Rowbotham, Samuel; Marjavaara, Lisette; et al.
S phase block following MEC1ATR inactivation occurs without severe dNTP depletion
Biology open, Vol. 4, (12) : 1739-1743
Earp, Caroline; Rowbotham, Samuel; Merényi, Gábor; et al.
Determination of deoxyribonucleoside triphosphate concentrations in yeast cells by strong anion-exchange high-performance liquid chromatography coupled with ultraviolet detection
DNA Replikation: Methods and Protocols, New York: Springer-Verlag New York 2015 : 113-121
Jia, Shaodong; Marjavaara, Lisette; Buckland, Robert; et al.
Colon cancer-associated mutator DNA polymerase δ variant causes expansion of dNTP pools increasing its own infidelity
Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, (19) : E2467-E2476
Mertz, Tony M; Sharma, Sushma; Chabes, Andrei; et al.
Evidence that processing of ribonucleotides in DNA by topoisomerase 1 is leading-strand specific
Nature Structural & Molecular Biology, Vol. 22, (4) : 291-U35
Williams, Jessica S; Clausen, Anders R; Lujan, Scott A; et al.
dNTP pool levels modulate mutator phenotypes of error-prone DNA polymerase ε variants
Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, (19) : E2457-E2466
Williams, Lindsey N; Marjavaara, Lisette; Knowels, Gary M; et al.
Visa publikationer i DiVA

Forskargrupper

Forskningsledare
Andrei Chabes Lab
Andrei Chabes: Kan sjukdomar botas med hjälp av DNAs byggstenar?

Brist på DNAs byggstenar i vilande celler, leder till sjukdomar hos både barn och vuxna.