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Staff photo Stefan Björklund
Press photo

Stefan Björklund

The mediator complex and its function in transcriptional regulation at the molecular level

Research qualifications: Docent

Contact

E-mail
stefan.bjorklund@umu.se
Phone
+46 70 216 28 90

Works as

Affiliation
Professor at Department of Medical Biochemistry and Biophysics
Location
KB. A6, Campus, Umeå Umeå universitet, 901 87 Umeå
The role of mediator subunit MED7 in Arabidopsis development
Frontiers in Plant Science, Frontiers Media S.A. 2025, Vol. 16
Kumar, Koppolu Raja Rajesh; Blomberg, Jeanette; Björklund, Stefan
Growth-regulated co-occupancy of Mediator and Lsm3 at intronic ribosomal protein genes
Nucleic Acids Research, Oxford University Press 2024, Vol. 52, (11) : 6220-6233
Abdel-Fattah, Wael R.; Carlsson, Mattias; Hu, Guo-Zhen; et al.
Pseudomonas syringae infectivity correlates to altered transcript and metabolite levels of Arabidopsis mediator mutants
Scientific Reports, Springer Nature 2024, Vol. 14, (1)
Blomberg, Jeanette; Tasselius, Viktor; Vergara, Alexander; et al.
CDK8 of the mediator kinase module connects leaf development to the establishment of correct stomata patterning by regulating the levels of the transcription factor SPEECHLESS (SPCH)
Plant, Cell and Environment, John Wiley & Sons 2024, Vol. 47, (12) : 5237-5251
Hermida-Carrera, Carmen; Vergara, Alexander; Cervela-Cardona, Luis Manuel; et al.
Specific functions for Mediator complex subunits from different modules in the transcriptional response of Arabidopsis thaliana to abiotic stress
Scientific Reports, Nature Publishing Group 2020, Vol. 10, (1)
Crawford, Tim; Karamat, Fazeelat; Lehotai, Nora; et al.
The MED7 subunit paralogs of Mediator function redundantly in development of etiolated seedlings in Arabidopsis
The Plant Journal, John Wiley & Sons 2018, Vol. 96, (3) : 578-594
Kumar, Koppolu Raja Rajesh; Blomberg, Jeanette; Björklund, Stefan
Mediator binds to boundaries of chromosomal interaction domains and to proteins involved in DNA looping, RNA metabolism, chromatin remodeling, and actin assembly
Nucleic Acids Research, Oxford University Press 2017, Vol. 45, (15) : 8806-8821
Chereji, Razvan V.; Bharatula, Vasudha; Elfving, Nils; et al.
Functional metabolomics as a tool to analyze Mediator function and structure in plants
PLOS ONE, Public Library Science 2017, Vol. 12, (6)
Davoine, Celine; Abreu, Ilka N.; Khajeh, Khalil; et al.
MEDIATOR18 and MEDIATOR20 confer susceptibility to Fusarium oxysporum in Arabidopsis thaliana
PLOS ONE, PUBLIC LIBRARY SCIENCE 2017, Vol. 12, (4)
Fallath, Thorya; Kidd, Brendan N.; Stiller, Jiri; et al.
Redox regulation of the MED28 and MED32 mediator subunits is important for development and senescence
Protoplasma, Vol. 253, (3) : 957-963
Shaikhali, Jehad; Davoine, Céline; Björklund, Stefan; et al.
The Msn2 mediated stress response: Survival based on "hedging your bet" and a dynamic interplay of transcription factor binding and nucleosome occupancy
Yeast, Wiley-Blackwell 2015, Vol. 32, (Suppl. 1) : S221-S222
Broach, James R; Bharatula, Vasudha; Chereji, Razvan; et al.
Biochemical and redox characterization of the mediator complex and its associated transcription factor GeBPL, a GLABROUS1 enhancer binding protein
Biochemical Journal, Vol. 468, (3) : 385-400
Shaikhali, Jehad; Davoine, Celine; Brännström, Kristoffer; et al.
Interaction Studies of the Human and Arabidopsis thaliana Med25-ACID Proteins with the Herpes Simplex Virus VP16-and Plant-Specific Dreb2a Transcription Factors
PLOS ONE, Vol. 9, (5) : e98575-
Aguilar, Ximena; Blomberg, Jeanette; Brännström, Kristoffer; et al.
A dynamic interplay of nucleosome and Msn2 binding regulates kinetics of gene activation and repression following stress
Nucleic Acids Research, Vol. 42, (9) : 5468-5482
Elfving, Nils; Chereji, Razvan V.; Bharatula, Vasudha; et al.
Functional studies of the yeast Med5, Med15 and Med16 mediator tail subunits
PLOS ONE, Public Library of Science (PLOS) 2013, Vol. 8, (8)
Larsson, Miriam; Uvell, Hanna; Sandström, Jenny; et al.
Interactions between DNA, transcriptional regulator Dreb2a and the Med25 mediator subunit from Arabidopsis thaliana involve conformational changes
Nucleic Acids Research, Oxford: Oxford University Press 2012, Vol. 40, (13) : 5938-5950
Blomberg, Jeanette; Aguilar, Ximena; Brännström, Kristoffer; et al.
The Arabidopsis thaliana Med25 mediator subunit integrates environmental cues to control plant development
Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, (20) : 8245-8250
Elfving, Nils; Davoine, Céline; Benlloch, Reyes; et al.
Med8, Med18, and Med20 subunits of the Mediator head domain are interdependent upon each other for folding and complex formation
Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, (49) : 20728-20733
Shaikhibrahim, Zaki; Rahaman, Hamidur; Wittung-Stafshede, Pernilla; et al.
Purification of a plant mediator from Arabidopsis thaliana identifies PFT1 as the Med25 subunit
Molecular Cell, Vol. 26, (5) : 717-729
Bäckström, Stefan; Elfving, Nils; Nilsson, Robert; et al.
Functional and physical interactions within the middle domain of the yeast mediator
Molecular Genetics and Genomics, Vol. 276, (2) : 197-210
Hallberg, Magnus; Hu, Guo-Zhen; Tronnersjö, Susanna; et al.
The yeast Mediator complex and its regulation.
Trends in Biochemical Sciences, Vol. 30, (5) : 240-4
Björklund, Stefan; Gustafsson, Claes M
The mediator complex.
Advances in Protein Chemistry, Vol. 67 : 43-65
Björklund, Stefan; Gustafsson, Claes M
A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II.
Molecular Cell, Vol. 14, (5) : 553-7
Bourbon, Henri-Marc; Aguilera, Andres; Ansari, Aseem Z; et al.
S Phase-specific transcription of the mouse ribonucleotide reductase R2 gene requires both a proximal repressive E2F-binding site and an upstream promoter activating region.
Journal of biological chemistry, Vol. 279, (11) : 10796-807
Chabes, Anna Lena; Björklund, Stefan; Thelander, Lars
Site-specific Srb10-dependent phosphorylation of the yeast Mediator subunit Med2 regulates gene expression from the 2-microm plasmid
Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, (10) : 3370-3375
Hallberg, Magnus; Polozkov, Gennady; Hu, Guo-Zhen; et al.
Functional interactions within yeast mediator and evidence of differential subunit modifications
Journal of Biological Chemistry, Vol. 278, (6) : 3831-3839
Balciunas, Darius; Hallberg, Magnus; Björklund, Stefan; et al.
Sequences downstream of the transcription initiation site are important for proper initiation and regulation of mouse ribonucleotide reductase R2 gene transcription
European Journal of Biochemistry, Blackwell Publishing 2003, Vol. 270, (8) : 1791-1801
Kotova, Irina; Chabes, Anna Lena; Lobov, Sergei; et al.
The yeast mediator
Molecules and Cells, Elsevier 2001, Vol. 11, (2) : 129-136
Björklund, Stefan; Buzaite, Odeta; Hallberg, Magnus
A mouse in vitro transcription system reconstituted from highly purified RNA polymerase II, TFIIH, and recombinant TBP, TFIIB, TFIIE and TFIIF.
European Journal of Biochemistry, Vol. 268, (16) : 4527-4536
Kotova, Irina; Chabes, Anna Lena; Segerman, Bo; et al.
The Med1 subunit of the yeast mediator complex is involved in both transcriptional activation and repression
Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences (PNAS) 1999, Vol. 96, (2) : 376-381
Balciunas, Darius; Gälman, Cecilia; Ronne, Hans; et al.
Global transcription regulators of eukaryotes
Cell, Elsevier 1999, Vol. 96, (6) : 759-767
Björklund, Stefan; Almouzni, Geneviève; Davidson, Irwin; et al.
Evidence for a mediator cycle at the initiation of transcription
Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences (PNAS) 1997, Vol. 94, (12) : 6075-6078
Svejstrup, Jesper Q.; Li, Yang; Fellows, Jane; et al.
Mediator of transcriptional regulation.
TIBS -Trends in Biochemical Sciences. Regular ed., Vol. 21, (9) : 335-7
Björklund, Stefan; Kim, Y J
Induction of the mouse ribonucleotide reductase R1 and R2 genes in response to DNA damage by UV light.
Journal of Biological Chemistry, Vol. 271, (39) : 23698-704
Filatov, D; Björklund, Stefan; Johansson, Erik; et al.
Yeast RNA polymerase II holoenzyme.
Methods in Enzymology, Vol. 273 : 172-5
Li, Y; Bjorklund, Stefan; Kim, Y J; et al.
Yeast global transcriptional regulators Sin4 and Rgr1 are components of mediator complex/RNA polymerase II holoenzyme.
Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, (24) : 10864-8
Li, Y; Bjorklund, Stefan; Jiang, Y W; et al.
Identification and characterization of a TFIID-like multiprotein complex from Saccharomyces cerevisiae.
Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, (18) : 8224-8
Poon, D; Bai, Y; Campbell, A M; et al.
Gene structure and regulation of the expression of the R1 and R2 subunits of mouse ribonucleotide reductase.
Advances in Experimental Medicine and Biology, Vol. 370 : 721-4
Johansson, Erik; Björklund, Stefan; Thelander, Lars
A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II.
Cell, Vol. 77, (4) : 599-608
Kim, Y J; Björklund, Stefan; Li, Y; et al.
Structure and promoter characterization of the gene encoding the large subunit (R1 protein) of mouse ribonucleotide reductase.
Proceedings of the National Academy of Science U S A, Vol. 90, (23) : 11322-6
Björklund, Stefan; Hjortsberg, K; Johansson, Erik; et al.
An S-phase specific release from a transcriptional block regulates the expression of mouse ribonucleotide reductase R2 subunit
EMBO Journal, Vol. 11, (13) : 4953-4959
Björklund, Stefan; Skogman, E; Thelander, Lars
S-phase-specific expression of mammalian ribonucleotide reductase R1 and R2 subunit mRNAs
Biochemistry, Vol. 29, (23) : 5452-5458
Björklund, Stefan; Skog, Sven; Tribukait, Bernard; et al.
S phase-specific transcription of the mouse ribonucleotide reductase R2 gene is dependent on an upstream promoter activating region and a proximal repressive E2F binding site.
Chabes, Anna Lena; Björklund, Stefan; Thelander, Lars
Mediator exists in multiple forms and is predominantly associated to promoters with low nuclesome density
Elfving, Nils; Chereji, Razvan; Larsson, Miriam; et al.
Mechanisms of transcriptional activation of the AdML promoter by NF-Y.
Frontini, M; Kotova, Irina; Björklund, Stefan; et al.
Functional and physical interactions of the Mediator subunit Med21/Srb7
Hallberg, Magnus; Hu, Guo-Zhen; Balciunas, Darius; et al.
Phosphorylation of Serine 208 in Med2 is important for proper expression of genes required for anaerobic growth and purine and glykogen metabolism
Hallberg, Magnus; Polozkov, Gennady; Deluen, Cécile; et al.
Med2 is essential for growth under anaerobic conditions and involved in expression of genes that are upregulated during anaerobiosis
Nilsson, Miriam; Hallberg, Magnus; Deluen, Cécile; et al.
View publications in DiVA

Research groups

CU4LS action group 2021
Coalition Umeå for Life Science (CU4LS)
Head of research
Stefan Björklund lab

Research projects

23 April 2003 until 31 December 2014
The function of Mediator in transcriptional regulation