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Umeå University
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Staff photo Sjoerd Wanrooij

Sjoerd Wanrooij

Molecular mechanism of DNA repair and replication in human mitochondria

Contact

E-mail
sjoerd.wanrooij@umu.se
Phone
+46 72 246 03 09

Works at

Affiliation
Associate professor at Department of Medical Biochemistry and Biophysics
Location
KB. A6, Campus, Umeå Umeå universitet, 901 87 Umeå

Human mitochondrial DNA (mtDNA) encodes for genes that are essential for proper energy production. Loss of mtDNA function results in a broad range of genetic human disorders, and it is increasingly recognized as a significant factor in common disorders such as neurodegeneration. Our group investigates detailed molecular mechanisms of mitochondrial DNA replication and repair in order to better understand the underlying causes of such disease states.

Exploring the dispersion and electrostatic components in arene-arene interactions between ligands and G4 DNA to develop G4-ligands
Journal of Medicinal Chemistry, American Chemical Society (ACS) 2024, Vol. 67, (3) : 2202-2219
Andréasson, Måns; Donzel, Maxime; Abrahamsson, Alva; et al.
G4-ligand-conjugated oligonucleotides mediate selective binding and stabilization of individual G4 DNA structures
Journal of the American Chemical Society, American Chemical Society (ACS) 2024, Vol. 146, (10) : 6926-6935
Berner, Andreas; Das, Rabindra Nath; Bhuma, Naresh; et al.
Preferential binding of ADP-bound mitochondrial HSP70 to the nucleotide exchange factor GRPEL1 over GRPEL2
Protein Science, John Wiley & Sons 2024, Vol. 33, (11)
Manjunath, Pooja; Stojkovic, Gorazd; Euro, Liliya; et al.
Unravelling the Therapeutic Potential of Antibiotics in Hypoxia in a Breast Cancer MCF-7 Cell Line Model
International Journal of Molecular Sciences, MDPI 2023, Vol. 24, (14)
Akhunzianov, Almaz A.; Nesterova, Alfiya I.; Wanrooij, Sjoerd; et al.
Enhanced mitochondrial G-quadruplex formation impedes replication fork progression leading to mtDNA loss in human cells
Nucleic Acids Research, Oxford University Press 2023, Vol. 51, (14) : 7392-7408
Doimo, Mara; Chaudhari, Namrata; Abrahamsson, Sanna; et al.
Rolling circle replication and bypass of damaged nucleotides
Mitochondrial DNA: methods and protocols, New York: Humana Press 2023 : 203-217
Forslund, Josefin M. E.; Stojkovič, Gorazd; Wanrooij, Sjoerd
TFB2M and POLRMT are essential for mammalian mitochondrial DNA replication
Biochimica et Biophysica Acta. Molecular Cell Research, Elsevier 2022, Vol. 1869, (1)
Inatomi, Teppei; Matsuda, Shigeru; Ishiuchi, Takashi; et al.
A complementary chemical probe approach towards customized studies of G-quadruplex DNA structures in live cells
Chemical Science, Royal Society of Chemistry 2022, Vol. 13, (8) : 2347-2354
Prasad, Bagineni; Doimo, Mara; Andréasson, Måns; et al.
Motif WFYY of human PrimPol is crucial to stabilize the incoming 3'-nucleotide during replication fork restart
Nucleic Acids Research, Oxford University Press 2021, Vol. 49, (14) : 8199-8213
Calvo, Patricia A; Martínez-Jiménez, María I; Díaz, Marcos; et al.
Human polymerase δ-interacting protein 2 (Poldip2) inhibits the formation of human tau oligomers and fibrils
International Journal of Molecular Sciences, MDPI 2021, Vol. 22, (11)
Kasho, Kazutoshi; Krasauskas, Lukas; Smirnovas, Vytautas; et al.
A unique arginine cluster in PolDIP2 enhances nucleotide binding and DNA synthesis by PrimPol
Nucleic Acids Research, Oxford University Press 2021, Vol. 49, (4) : 2179-2191
Kasho, Kazutoshi; Stojkovic, Gorazd; Velázquez-Ruiz, Cristina; et al.
MtDNA replication, maintenance, and nucleoid organization
The human mitochondrial genome: from basic biology to disease, Academic Press 2020 : 3-33
Doimo, Mara; Pfeiffer, Annika; Wanrooij, Paulina H.; et al.
Quinazoline Ligands Induce Cancer Cell Death through Selective STAT3 Inhibition and G-Quadruplex Stabilization
Journal of the American Chemical Society, Vol. 142, (6) : 2876-2888
Jamroskovic, Jan; Doimo, Mara; Chand, Karam; et al.
Photoactivated Colibactin Probes Induce Cellular DNA Damage
Angewandte Chemie International Edition, Vol. 58, (5) : 1417-1421
Moodie, Lindon W. K.; Hubert, Madlen; Zhou, Xin; et al.
A two-nuclease pathway involving RNase H1 is required for primer removal at human mitochondrial OriL
Nucleic Acids Research, Oxford University Press 2018, Vol. 46, (18) : 9471-9483
Al-Behadili, Ali; Uhler, Jay P.; Berglund, Anna-Karin; et al.
The presence of rNTPs decreases the speed of mitochondrial DNA replication
PLOS Genetics, Public library science 2018, Vol. 14, (3)
Forslund, Josefin M. E.; Pfeiffer, Annika; Stojkovič, Gorazd; et al.
Known Unknowns of Mammalian Mitochondrial DNA Maintenance
Bioessays, Wiley-Blackwell 2018, Vol. 40, (9)
Pohjoismäki, Jaakko L. O.; Forslund, Josefin; Goffart, Steffi; et al.
Optimization of the expression, purification and polymerase activity reaction conditions of recombinant human PrimPol
PLOS ONE, PUBLIC LIBRARY SCIENCE 2017, Vol. 12, (9)
Boldinova, Elizaveta O.; Stojkovic, Gorazd; Khairullin, Rafil; et al.
DNA Damage Tolerance by Eukaryotic DNA Polymerase and Primase PrimPol
International Journal of Molecular Sciences, MDPI AG 2017, Vol. 18, (7)
Boldinova, Elizaveta O.; Wanrooij, Paulina H.; Shilkin, Evgeniy S.; et al.
PrimPol is required for replication reinitiation after mtDNA damage
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences 2017, Vol. 114, (43) : 11398-11403
Torregrosa-Muñumer, Rubén; Forslund, Josefin M. E.; Goffart, Steffi; et al.
Ribonucleotides incorporated by the yeast mitochondrial DNA polymerase are not repaired
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences 2017, Vol. 114, (47) : 12466-12471
Wanrooij, Paulina H.; Engqvist, Martin K. M.; Forslund, Josefin M. E.; et al.
Oxidative DNA damage stalls the human mitochondrial replisome
Scientific Reports, Vol. 6
Stojkovic, Gorazd; Makarova, Alena V.; Wanrooij, Paulina H.; et al.
ClpX stimulates the mitochondrial unfolded protein response (UPRmt) in mammalian cells
Biochimica et Biophysica Acta. Molecular Cell Research, Vol. 1853, (10) : 2580-2591
Al-Furoukh, Natalie; Ianni, Alessandro; Nolte, Hendrik; et al.
In vivo occupancy of mitochondrial single-stranded DNA binding protein supports the strand displacement mode of DNA replication
PLOS Genetics, Vol. 10, (12) : e1004832-
Miralles Fusté, Javier; Shi, Yonghong; Wanrooij, Sjoerd; et al.
The Synergism of the Dispersion and Electrostatic Components in the Arene-Arene Interactions Between Ligands and G4 DNA
Andréasson, Måns; Donzel, Maxime; Abrahamsson, Alva; et al.
Development of a G4 Ligand-Conjugated Oligonucleotide Modality that Selectively Targets Individual G4 DNA Structures
Das, Rabindra Nath; Berner, Andreas; Bhuma, Naresh; et al.
View publications in DiVA

Research groups

Head of research
Sjoerd Wanrooij lab