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Umeå University
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Staff photo Paulina Wanrooij

Paulina Wanrooij

I lead a research group that focuses on mitochondrial DNA and the molecular mechanisms that mediate the signaling of its instability.

 

Research qualifications: Docent
0000-0002-8607-7564

Contact

E-mail
paulina.wanrooij@umu.se
Phone

Works at

Affiliation
Research fellow at Department of Medical Biochemistry and Biophysics
Location
KB. H6, Campus, Umeå Umeå universitet, 901 87 Umeå

Our cells contain genetic information in two separate compartments - the nucleus and the mitochondria. Mitochondrial DNA (mtDNA) is a small circular double-stranded DNA molecule that contains information that is essential for efficient energy production. Problems with mitochondrial DNA can therefore lead to a cellular energy defect and mitochondrial disease.

My group works on various aspects of mitochondrial DNA stability, and on how problems with mitochondrial DNA are signalled to the rest of the cell. A thorough understanding of the molecular mechanisms that mediate these signals relates to various hereditary mitochondrial diseases, but also to neurodegenerative disease and various types of cancers.

Read more about our research on www.pwanrooijlab.com

PrgE: an OB-fold protein from plasmid pCF10 with striking differences to prototypical bacterial SSBs
Life Science Alliance, Life Science Alliance 2024, Vol. 7, (8)
Breidenstein, Annika; Lamy, Anaïs; Bader P.J., Cyrielle; et al.
Mitochondrial membrane potential acts as a retrograde signal to regulate cell cycle progression
Life Science Alliance, Life Science Alliance, LLC 2023, Vol. 6, (12)
Gorospe, Choco Michael; Carvalho, Gustavo; Herrera Curbelo, Alicia; et al.
Determination of the ribonucleotide content of mtDNA using alkaline gels
Mitochondrial DNA: methods and protocols, New York: Humana Press 2023 : 293-314
Gorospe, Choco Michael; Repolês, Bruno Marçal; Wanrooij, Paulina H.
NME6: ribonucleotide salvage sustains mitochondrial transcription
EMBO Journal, John Wiley & Sons 2023, Vol. 42, (18)
Wanrooij, Paulina H.; Chabes, Andrei
Mitochondrial DNA Instability in Mammalian Cells
Antioxidants and Redox Signaling, Mary Ann Liebert 2022, Vol. 36, (13-15) : 885-905
Carvalho, Gustavo; Repolês, Bruno Marçal; Mendes, Isabela; et al.
The integrity and assay performance of tissue mitochondrial DNA is considerably affected by choice of isolation method
Mitochondrion (Amsterdam. Print), Elsevier 2021, Vol. 61 : 179-187
Repolês, Bruno Marçal; Gorospe, Choco Michael; Tran, Phong; et al.
MtDNA replication, maintenance, and nucleoid organization
The human mitochondrial genome: from basic biology to disease, Academic Press 2020 : 3-33
Doimo, Mara; Pfeiffer, Annika; Wanrooij, Paulina H.; et al.
Elimination of rNMPs from mitochondrial DNA has no effect on its stability
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences 2020, Vol. 117, (25) : 14306-14313
Wanrooij, Paulina H.; Tran, Phong; Thompson, Liam J.; et al.
De novo dNTP production is essential for normal postnatal murine heart development
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology 2019, Vol. 394, (44) : 15889-15897
Tran, Phong; Wanrooij, Paulina H.; Lorenzon, Paolo; et al.
Ribonucleotides in mitochondrial DNA
FEBS Letters, John Wiley & Sons 2019, Vol. 593, (13) : 1554-1565
Wanrooij, Paulina H.; Chabes, Andrei
The presence of rNTPs decreases the speed of mitochondrial DNA replication
PLOS Genetics, Public library science 2018, Vol. 14, (3)
Forslund, Josefin M. E.; Pfeiffer, Annika; Stojkovič, Gorazd; et al.
Inosine Triphosphate Pyrophosphatase Dephosphorylates Ribavirin Triphosphate and Reduced Enzymatic Activity Potentiates Mutagenesis in Hepatitis C Virus
Journal of Virology, American Society for Microbiology 2018, Vol. 92, (19)
Nystrom, Kristina; Wanrooij, Paulina H.; Waldenstrom, Jesper; et al.
DNA Damage Tolerance by Eukaryotic DNA Polymerase and Primase PrimPol
International Journal of Molecular Sciences, MDPI AG 2017, Vol. 18, (7)
Boldinova, Elizaveta O.; Wanrooij, Paulina H.; Shilkin, Evgeniy S.; et al.
Inosine triphosphate pyrophosphatase enhances the effect of ribavirin on hepatitis C virus cell culture infection
Journal of Hepatology, ELSEVIER SCIENCE BV 2017, Vol. 66, (1) : S321-S321
Nystrom, K.; Pettersson, G.; Wanrooij, Paulina; et al.
Ribonucleotides incorporated by the yeast mitochondrial DNA polymerase are not repaired
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences 2017, Vol. 114, (47) : 12466-12471
Wanrooij, Paulina H.; Engqvist, Martin K. M.; Forslund, Josefin M. E.; et al.
The dimeric architecture of checkpoint kinases Mec1(ATR) and Tel1(ATM) reveal a common structural organization
Journal of Biological Chemistry, Elsevier 2016, Vol. 291, (26) : 13436-13447
Sawicka, Marta; Wanrooij, Paulina H.; Darbari, Vidya C.; et al.
Oxidative DNA damage stalls the human mitochondrial replisome
Scientific Reports, Vol. 6
Stojkovic, Gorazd; Makarova, Alena V.; Wanrooij, Paulina H.; et al.
Probing the Mec1ATR Checkpoint Activation Mechanism with Small Peptides
Journal of Biological Chemistry, Vol. 291, (1) : 393-401
Wanrooij, Paulina H.; Tannous, Elias; Kumar, Sandeep; et al.
View publications in DiVA

Research groups

Head of research
Paulina Wanrooij lab

Research projects

1 January 2020 until 31 December 2022
Checkpoint mechanisms that safeguard the mitochondrial genome