Umeå transgene core facility

Sleepy baby rats

Image

Overview of the different technologies

Genotyping

Client responsibilities

• provide tissue samples or prepared DNA

• provide primer sequences and protocols for each PCR

• provide ordering form

UTCF responsibilities

• prepare DNA (if client has not)

• run PCR and visualize the result with agarose gel electrophoresis

• send result by email, complete with gel picture within 5 working days after we receive clients samples and ordering form

Embryo transfer

Client responsibilities:

• consult with UTCF coordinator

• for re-derivation of a mouse strain, provide at least 5 mating pairs for egg donation

UTCF responsibilities:

• Dissect oviducts in order to obtain fertilized eggs

• implant embryos into foster females, as many as possible from the number of eggs provided

• Provide at least one born litter from the foster females

For order of Embryo transfer always consult with the UTCF coordinator.

In Vitro Fertilization (IVF)

Client responsibilities:

• Consult with UTCF coordinator

• Provide frozen sperm, or male(s) from which sperm will be collected

• Provide females for superovulation

UTCF responsibilities:

• Collect eggs from 10-20 B6/CBA females, or other background

• Perform IVF using the provided sperm

• Perform embryo transfer with the fertilized embryos, up to 6 embryo transfers/strain

• House born pups until weaning

Pronuclear injection

Is nowadays not performed by UTCF, but we can help with the contact with another facility.

Sperm cryopreservation

Client responsibilities:

• Consult with the UTCF coordinator

• Provide 2 males, age 10 weeks to 6 months

• Pay a non-refundable start-up fee of SEK 1,000 (20% of total cost)

UTCF responsibilities:

• Freeze sperm from the males, 10 straws/male

• Perform an IVF as a quality control of the frozen sperm, 1 straw/male will be used

• Perform embryo transfer after IVF to ensure that live pups can be produced

• Store the frozen sperm in liquid nitrogen

Price list

Embryo transfer:                             SEK  7,800
In vitro fertilization:                         SEK  7,800 
Cryopreservation of sperm:              SEK   6,000 
annual freezer cost                          SEK      600 
Genotyping: cost per hour:               SEK      530 + reagents and consumables. Estimated time for 30 PCR samples =3,5-4 hours

Note, the prices apply to researchers affiliated to Umeå University.

Order forms

Genotyping

Questions?

More information at our information site.