Combining high-throughput methods with the power of single-cell for genome-wide probing of gene functions.
I am a molecular biologist currently working to obtain my PhD in Johan Henriksson lab. I have a BSc in Biology with specialization in Molecular Biology from the University of Vienna and a MSc Molecular Biology from Umeå University.
Despite the fact that Escherichia coli is one of the most studied and described bacteria to date, even the most E. coli strain most commonly used in laboratories nowadays, K12, still has more than 30 percent of its gene annotation not experimentally validated. This highlights the need for new, high-throughput methods for exploring bacterial gene functions.
My current project in involves unlocking perturbation sequencing, which is a single-cell method for genome-wide probing of gene functions in eukaryotic cells, for prokaryotes. Such a method would allow us to explore the function of all genes in a bacterial genome at once, for any lab-cultivatable strain.