Research infrastructure Flow cytometry enables simultaneously analysis of cell surface bound as well as intracellular proteins and RNA expression on a single cell basis. In addition, secreted proteins can be analyzed using a bead based systems.
Flow cytometry is a powerful tool for rapid analysis of single cells or particles. It allows for distinct phenotyping of cells based on their expression of surface- or intracellular molecules or proteins. The additional capacity for rapid cell sorting based on these characteristics also allows for downstream applications such as functional- or genetic/transcriptional analysis. The use of flow cytometry to measure fluorescently labelled beads in a multiplex setting also allows for simultaneous assessment of hundreds of biomarkers in one sample.
The Umeå University Flow Cytometric Core Facility provides state-of-the-art equipment and training for advanced analysis and cell sorting of single cells or detection of multiple biomarkers in solution. We can also aid with hands-on sample and equipment handling, if required.
Available equipment:
|
APPLICATION |
EQUIPMENT |
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Phenotypic single cell analysis |
5-laser Biorad ZE5 cell analyzer |
|
Bulk or single cell sorting |
3-laser BD Melody (BSL-2 cell sorter) |
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Multiplex for biomarker analysis |
BioRad Bioplex 200 |
|
ELISpot / Fluorospot |
Mabtech IRIS 4 color Fluorospot |
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High capacity liquid handling |
Hamilton Microlab Star automated pipetting robot |
