BMCU provides access to two techniques for the characterization of biomolecules.
BMCU currently provides access to two techniques for the characterization of biomolecules: circular dichroism can be used to study protein conformations; mass photometry is a simple and versatile techniques to characterize the size of biomolecules or of biomolecular assemblies.
For further information, you can contact Johan Olofsson Edlund, or the person responsible for the instrument you are interested in (listed below together with the instrument).
ImageJohan
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Documentation
J-1700 CD Spectrometer
Location
Room A52907
Directions using digital map
Price
Full 24 hours – 800kr
8 hours during the day – 600kr
6 hours during the day – 500kr
4 hours during the day – 400kr
2 hours during the day – 300kr
Odd hours 18-08 - 300kr/booking
Introduction/drivers license (2hours) - 1000kr
Contact
Johan Olofsson Edlund
Ikenna Obi
About
Circular dichroism (CD) is a form of light absorption spectroscopy that measures the difference in absorbance of right- and left-circularly polarized light. This technique is most commonly used to study protein conformations and for structural analysis of biomolecules.
Some features of our CD instrument:
ImageMalvern Panalyticals
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Manual/Documentation
NanoSight NS300
Location
Room B-524b
Directions using a digital map
Price
100 per hour
Introduction/driver license (1 day - two sessions of approx. 3 hours) – 2500 kr
Contact
Birendra Singh (birendra.singh@umu.se)
Jonathan Gilthorpe (jonathan.gilthorpe@umu.se)
About
Particles suspended in a liquid are loaded into the laser module sample chamber and viewed in close proximity to the optical element. The NS300 device illuminates the particles using a specially aligned and focused laser beam. This allows extremely small particles (down to 10 nm, dependent on refractive index, while, typically for biological samples, the lower limit is around 40 mn) to be seen directly and individually by conventional microscopy. Particles in the liquid sample which pass through the beam path are seen as small points of light moving rapidly under Brownian motion, allowing information on particle properties to be obtained. With the NS300, you can analyze the presence, size distribution, and concentration of all types of nanoparticles from 10 nm to 2000 nm, depending on the instrument configuration and sample type. With the use of a suitable filter, fluorescent particles can also be analyzed. The instrument is equipped with a 488 nm laser for excitation and a filter set for green emission (510 nm – e.g. GFP or Alexa 488). The laser module contains thermoelectric Peltier elements, allowing the sample temperature to be controlled. This is fully programmable using the NTA software suite.
Mass Photometer, BioMolecular Characterization Umeå, BICU
ImageRonnie BerntssonMore details
Location
A6-52-21 KBC Fysiologihuset, Plan 6
Price
100 SEK /hour, 500 SEK/day and 800 SEK for introduction (2 hours)
Contact
Farahnez Ranjibairan
Johan Olofsson Edlund johan.olofsson.edlund@umu.se
About
Mass photometry (MP) is a light scattering based technique that allows the detection of unlabeled, individual molecules in diluted samples. The machine allows for detection of masses between 40 kDa up to ca 5 MDa. In addition, you get information about the relative abundance of species.
The end result is a graph showing the distribution of molecular masses in the sample. The molecule that is being studied thus needs to be purified, but only very small amounts of it is actually needed for the experiment.