Biomolecular characterization

BMCU currently provides access to two techniques for the characterization of biomolecules: circular dichroism can be used to study protein conformations; mass photometry is a simple and versatile techniques to characterize the size of biomolecules or of biomolecular assemblies.

For further information, you can contact Johan Olofsson Edlund, or the person responsible for the instrument you are interested in (listed below together with the instrument).

J-1700 CD Spectrometer

 

ImageJohan

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Documentation
J-1700 CD Spectrometer

Location
Room A52907
Directions using digital map

Price
Full 24 hours – 800kr
8 hours during the day – 600kr
6 hours during the day – 500kr
4 hours during the day – 400kr
2 hours during the day – 300kr
Odd hours 18-08 - 300kr/booking
Introduction/drivers license (2hours) - 1000kr

Contact
Johan Olofsson Edlund
Ikenna Obi

About
Circular dichroism (CD) is a form of light absorption spectroscopy that measures the difference in absorbance of right- and left-circularly polarized light. This technique is most commonly used to study protein conformations and for structural analysis of biomolecules. 

Some features of our CD instrument:

• Wavelength range 163 - 2500nm
• Simultaneous Multi-Probe (SMP): CD, LD, fluorescence, UV-visible, absorbance
• Three scanning modes: auto, step and continuous
• A carousel for 6 cuvettes allows measurements of 6 samples at the time
• A thermoregulation module is available and allows for temperature gradient measurements

Refeyn TwoMP – Mass Photometry

Mass Photometer, BioMolecular Characterization Umeå, BICU

ImageRonnie Berntsson

More details

Location
A6-52-21 KBC Fysiologihuset, Plan 6

Price
100 SEK /hour, 500 SEK/day and 800 SEK for introduction (2 hours)

Contact
Farahnez Ranjibairan
Johan Olofsson Edlund johan.olofsson.edlund@umu.se

About
Mass photometry (MP) is a light scattering based technique that allows the detection of unlabeled, individual molecules in diluted samples. The machine allows for detection of masses between 40 kDa up to ca 5 MDa. In addition, you get information about the relative abundance of species.

The end result is a graph showing the distribution of molecular masses in the sample. The molecule that is being studied thus needs to be purified, but only very small amounts of it is actually needed for the experiment.