Light Microscopy

Within light microscopy we provide advanced live cell imaging techniques including FLIM, FRAP, FLIM-FRET, TIRF, SIM, SMLM, Spinning disk confocal and 4D confocal.

Contact:

Light Microscopy Booking:

To book any of our instruments, please visit our Online Booking System

Note: The e-booking requires that you have had previous education on the specific system. If you dont have access to e-booking or if you want education or support please contact Irene Martinez

Rules:

• To operate the instruments on your own, you must have a licence. The licence will allow you to access the e-booking system and the server
• Data should be transferred to the server and to your personal storage. You cannot store your images in the server or the microscope computer.
• For funding purposes, it is essential to acknowledge BICU in all publications that include data derived from the facility. Include this statement: "Microscopy was performed at the Biochemical Imaging Centre Umeå.

Light Microscopy Instruments and Workstations

Leica SP8 FALCON Confocal

More Details

• Location:
  Chemistry Department 4th floor corridor A4
• Room number:
  A4.41.01
• Price:
  250kr/hr - Driver's license 2000kr
• Objectives:
  • HC PL FLUOTAR 10x/0.30
  • HC PL APO 20x/0.75 CS2
  • HC PL APO 63x/1.20 Water motCORR CS2
  • HC PL APO 63x/1.40 OIL CS2
• Laser Lines:
  • Diode 405nm
  • Argon Laser (458nm, 476nm, 488nm, 496nm and 514nm)
  • WLL (White light laser). Tunable excitation from 470 to 670nm in steps of 1nm
• Techniques:
  • Confocal Imaging
  • FLIM (biosensing, species separation, FLIM-FRET)
  • FRAP
• Other specifications:
  • One PMT detector, two HyD SP GaAsP detectors and one HyD SP generation2 detector optimized for FLIM
  • Motorized Focus with AFC
  • Resonant Scanner 12 kHz
  • Live cell incubation
  • Microinjector
     

Leica SP8 Confocal

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• Location:
  Molecular Biology
• Room number:
  6L-021
• Price:
  150 kr/hr - Driver's license 2000kr
• Objectives:
  • HC PL APO 20x/0.75 Dry
  • HC PL APO 40x/1.30 Oil CS2
  • HC PL APO 63x/1.40 OIL CS2
• Laser Lines:
  Diode 405nm, Diode 638nm, OPSL 488nm, OPSL 552nm
• Techniques:
  • Confocal Imaging
  • Resonant Scanner 8 kHz

Zeiss Spinning Disk Confocal

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Zeiss Cell Observer Spinning Disk Confocal, equipped with two EMCCD cameras to perform confocal and TIRF microscopy.

• Location:
  Department of Medical and Translational Biology (MTB), 5thfloor
• Room:
  D2-524
• Price:
  250kr/hr - Driver's license 2000kr
• Objectives:
  20X (NA 0.8); 40X W (NA 1.2); 63X oil (NA 1.40) DIC; 100X Tirf oil (NA 1.46) DIC
• Laser lines:
  405nm, 488nm, 561nm and 647nm

• Techniques:
  • Confocal imaging (1000 frames/sec)
  • TIRF microscopy

• Other specifications:
  • Live cell incubationDefinite Focus system
  • Direct FRAP
  • Microfuidics
• Manual for Download:
  • Spinning Disk

Zeiss Elyra 7

More Details

• Location:

MTB: Department of Medical and Translational Biology, 5th floor

• Room Number:

D2-524

• Price:

Please, contact Irene Martinez to consult the Elyra prices

• Objectives

EC Plan-Neofluar 10x/0.30 WD=5.2 M27

Objective PApo 20x/0.8

Plan-Apochromat 40x/0.95 Corr D=0.25 M27

Plan-Apochromat 63x/1.40 Oil DIC f/ELYRA

Objective alpha PApo 63x/1.46 Oil Corr

• Laser lines:

405nm, 488nm (100mW), 561nm (100mW), 640nm (500mW)

• Techniques:

ApoTome

Total Internal Reflection Fluorescence Microscopy (TIRF)

Structured Illumination Microscopy (SIM)

Single Molecule Localization Microscopy (SMLM)

• Other specifications:

2 CMOS-cameras PCO.edge 4.2 CLHS adjusted with DuoLink

Live cell imaging incubation

Nikon A1R Confocal

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Nikon A1R confocal (LSM) controlled by Nikon NIS Elements interface with a Nikon Eclipse Ti-E inverted microscope.

• Location:
  KBC Building 6thfloor Department of Medical Biochemistry and Biophysics.
• Room:
  A6 44 15
• Price:
  250kr/hr - Driver's license 2000kr (confocal microscopy)
  150kr/hr - Driver's license 2000kr (widefield microscopy)

• Objectives:
  10X (NA 0.45) DIC 20X (NA 0.75) DIC 60X oil (NA 1.40) DIC 100X Tirf oil (NA 1.49) DIC
• Laser lines:
  405nm, 457, 477, 488, 514nm, 561nm and 647nm
• Techniques:
  • Confocal microscopy (30 frames/sec)
  • Full spectral resolution (spectral and conventional detectors)
  • STORM microscopy
  • TIRF microscopy
  • Epifluoresence microscopy
• Other specifications:
  • Live cell incubation
  • Micromanipulators
  • Perfect focus system
• Manuals for Download:
  • Nikon A1R Widefield
  • Nikon A1R Confocal

 

Leica Widefield Thunder

More Details

• Location:
  KBC Building 6th floor Department of Medical Biochemistry and Biophysics.
• Room:
  A6 44 15
• Price:
  150kr/hr - Driver's License 2000kr
• Objectives:
  • N PLAN 5x/0.12 PH0
  • HC PL FLUOT AR 10x/0.32 PH1
  • HC PL FL L 20x/0.40 CORR PH1
  • HC PL APO 20x/0.80
  • HC PL FLUOTAR L 40x/0.60 CORR
  • HC PL APO 40x/0.95 CORR
  • HC PL APO 63x/1.40-0.60 OIL
  • HC PL FLUOTAR 100x/1.32 OIL PH3
• Techniques:
  • Widefield
  • Live cell imaging
  • Computational clearance: Thunder

Nikon Widefield/Tirf

More Details

• Location:
  Department of Medical and Translational Biology, MTB, 5th floor
• Room:
  D2-5th floor