Atomic Force Microscopy

Introduction

Our AFM can be used to scan various surfaces ranging from metal, glass, and surfaces coated with biomolecules to live cells. It can be used to extract mechanical information such as adhesion, stiffness, and deformation. In addition, our AFM can precisely measure the bond strength and binding affinities of biomolecular interactions.

Contact

Fouzia Bano Fouzia.bano@umu.se

Booking

To book any of our instruments, please visit our Online Booking System
Note:The e-booking requires that you have had previous education on the specific system. If you dont have access to e-booking or if you want education or support, please contact Fouzia Bano

You can add the second image attached here before the “More Details”

More Details

• Location:
  NUS, building 6M
• Room:
  06M-RUM-6098
• Prices:
  • Driver’s license (experienced user): 2500 kr
  • Driver’s license (beginner, Imaging only: 5000 kr
  • Driver’s license (beginner, Force spectroscopy 7500 kr)
  • Instrument usage without assistance: 200 kr per hour
  • Instrument usage with assistance: 650 kr per hour
  • Cantilever (if the user hasn’t yet bought their own): 1000 SEK/cantilever.
  • Email us to check the availability of the type of cantilevers.
• Specifications:
  • Nanowizard® 4 XP BioScience (JPK/Bruker) equipped with motorized sample stage and top view optical module.
  • Possibility to combine it with Nikon Widefield light microscope
  • XY scanner range: 100x100 µm2
  • Z scanner range: 3.5 to15 µm
  • BioCell® with temperature and CO2 control for life science imaging
  • JPK acoustic enclosure with base table and tabletop foam enclosure
  • Located at BSL2 working environment
• Major operating modes:
  • Imaging modes
    • Contact and Tapping Mode™
    • PeakForce Tapping
    • Advanced Quantitative Imaging (QI™) (see nanomechanics)
  • Force measurements
    • Standard contact mode force spectroscopy
    • Advanced force mapping (RampDesigner™ for custom-designed clamp and ramp experiments)
  • Nanomechanics
    • QI™ mode to acquire simultaneously topographic features and mechanical properties such as adhesion, elasticity, stiffness, and deformation of samples ranging from hard glass to soft cells/tissues
  • Major applications:
    • Imaging of biomolecules such as proteins, DNA, bacteria, and viruses on the surface
    • Live cell imaging and mechanics
    • Single-molecule force spectroscopy
    • Simultaneous AFM and fluorescence imaging of cells and tissues
  • The host lab is currently using this instrument in the BSL2 facility (6M) to probe the biomolecular interactions such as carbohydrates and viruses and to scan the various stage of live cells upon infection with viruses under physiological conditions. 
• More information: 
  • https://www.bruker.com/en/products-and-solutions/microscopes/bioafm/jpk-nanowizard-4-xp-bioscience.html
  • For AFM handbook, click here.