Infrastructure at TRC

Biosensing

Biacore

Modell: T100.
Surface plasmon resonance allows real-time, label-free detection of biomolecular interactions.
Contact: Lars Frängsmyr, 070-2765041

QCM-D

Contact: Marta Bally

 

Cell culture

HoloMonitor M4

Phase Holographic Imaging.
Contact: Annika Holmström

Hypoxia incubator

Modell: Galaxy 48 R.
N2 not routinely supplied.
Contact: Lotta Nordström

Orgaoid Lab

3D culturing of cells in matrigel. You can purchase Matrigel vials (1 ml or10ml), r-spondin conditioned media (10ml) and wnt-conditioned media (10 ml).
Contact: Mitesh Dongre, 090-785 17 28

 

Centrufuger

Ultracentrifuge

Contact: Emma Nilsson, 070-5159528

High-speed centrifuge

Contact: Emma Nilsson, 070-5159528

 

ddPCR

Droplet generator.

Modell: QX200. 
Contact: Jonas Nilsson, 090-785 85 61

Droplet reader

Modell: Q200. 
Contact: Jonas Nilsson, 090-785 85 61

ddSEQ

Single-cell isolator.
Contact: Jonas Nilsson, 090-785 85 61

 

Flödescytometri

BDLSRII

Modell: BDLSRII.
Cell analysis.
Contact: Kristina Lejon, 090-785 35 76

Accuri

Modell: Accuri.
Cell analysis.
Contact: Kristina Lejon, 090-785 35 76

Luminex

Modell: Luminex.
Bead array. 
Contact: Kristina Lejon, 090-785 35 76

BDAriaIII

Modell: BDAriaIII.
Cell sorter.
Contact: Kristina Lejon, 090-785 35 76

ZetaView

Video microscop to analyze nanoparticles.
Contact: Marta Bally, Konrad Thorsteinsson, 070-2158653

autoMACS pro

Magnetic, automated cell sorter.
Contact: Sofie Degerman, 090-785 28 73

 

Labeling

Label printer

Modell: PM43c.
Contact: Emma Honkala

 

Histology

Leica CM3050 S Research Cryostat

Modell: CM3050S.
Cryostat.
Contact: Anette Berglund, 090-785 17 26

Benchmark ULTRA

Automated IHC stainer. 
Contact: Pernilla Andersson, 090-785 28 33

Benchmark ULTRA #2

Automated IHC stainer. 
Contact: Anette Berglund, 090-785 17 26 

TMA Grand Master

Tissue microarray puncher.
Contact: Susanne Gidlund

 

Imaging

Gel documentation

Contact: Emma Nilsson, 090-785 28 79

GelDoc XR+

Gel documentation.
Modell: Imager 600
Contact: Maria Brattsand, 090-785 29 95

Azure c600 imaging system

Modell: c600
Imaging system for gels and membranes. Chemiluminescence, RGB, NIR and UV.
Contact: Ola Billing, 090-785 69 85

Panoramic 250 scanner

Whole tissue slide brightfield scanner.
Contact: Susanne Gidlund

 

Microscopy

Zeiss Axiovert

Modell: Axiovert
Contact: David Persson

Zeiss Axioskop 2

Modell: Axioskop2
Contact: David Persson

Nikon eclipse Ti2

Modell: Eclipse Ti2.
Inverted epifluorescence, TIRF illumination.
Contact: Marta Bally, Konrad Thorsteinsson, 070-2158653

Evos FL

4x, 10x, 20x objectives. Visible/ Fluorescence.
Contact: Mitesh Dongre, 090-785 17 28

Zeiss Axiozoom v16

Zoom/stereo microscope. LED UV light source. Green, red and dual green/red long pass filter.
Contact: Ola Billing, 090-785 69 85

Olympus BX51

Modell: BX51.
Compound microscope. Equipped with DIC contrast and UV with DAPI, FITC and TRITC filters.
Contakt: Ola Billing, 090-785 69 85

PALM Microbeam

Laser capture microdissection.
Contact: Pernilla Wikström, 090-785 37 52

VECTRA 3 Quantitative Pathology Imaging System

Multispectral imaging. DAPI, FITC, CY3, Texas Red, and CY5 filters.
Contact: Richard Palmqvist, 090-786 91 92, 070-645 12 50

 

PCR

Q-PCR

Modell: Step One Plus.
Contact: Islam Koushikul

QuantStudio 6 Flex 2 Red/Blue

Real-time PCR
Contact: Sofie Degerman, 090-785 28 73

2x Q-PCR

Modell: QuantStudio5.
Contact: Richard Lindqvist

 

Pipetting

Biomek 4000

Pipetting robot, liquid handling.
Contact: Mikael Kimdal, 090-785 29 18

 

Plate readers

GloMax 96 microplate luminometer

Contact: Håkan Hedman, 090-785 28 81

SpectroMax I3

Microscopy/ spectroscopy. Compatible with differnet plate types.
Contact: James Mason, 090-785 17 28

Varioscan Flash

Plate reader.
Contact: Marta Bally, Konrad Thorsteinsson, 070-2158653

Tecan plate reader

Modell: Infinite F2000pro.
Also works as ELISA reader
Contact: Mårten Strand 

BMG Fluostar Omega

Modell Fluostar Omega. Fluorescence plate reader with C02 regulation.
Contact: Nathalie Uwamahoro, 0724520353

 

Sequencing

MinION nanopore sequencer

Long-read DNA/RNA sequencing
Contact: Jonas Nilsson, 090-785 85 61

HiScan

Illumina sequencing and microarray-based analysis. SNP-genotyping, gene expression profiling and methylation analysis.
Contact: Sofie Degerman, 090-785 28 73

 

Spectrophotometry

Bioanalyzer 2100

Modell: G2939A.
Bioanalyzer is a chip-based capillary electrophoresis machine to analyse RNA, DNA, and protein: All consumables are for sale.
Contact: Cedric Patthey, 070-534 85 26

Nanodrop

Modell: ND-1000.
Contact: Håkan Hedman, 090-785 28 81

 

Tissue prep

CryoXtractor

Modell: CXT350.
Extraction of frozen biospecimens.
Contact: Erik Bovinder Ylitalo

Precellys 24 tissue homogenizer

High-throughput bead mill homogenizer
Contact: Håkan Hedman, 090-785 28 81

FastPrep24

Modell: 24. Tissue homogenizer.
Contact: Jan Olsson, 070-5133378

NorDiag Arrow robot

DNA/RNA preparation.
Contact: Jan Olsson, 070-5133378

gentleMACS Octo Dissociator

Semi-automated tissue dissociation and homogenization.
Contact: Sofie Degerman, 090-785 28 73

 

Water/ice

Ice machine

Contact: Anne Israelsson, 073-8415180

Ice machine

Contact: Clas Wikström, 070-3834488

MQ-H2O

Modell: Millipore. Highly pure water, only small volumes.
Contact: Anne Israelsson, 073-8415180

MQ-H2O

Modell: ELGA. Deionized water.
Contact: Nathalie Uwamahoro, 0724520353